Wie verwende ich ordnungsgemäß codonbias.scores.Frequencyoptimalcodons? ⇐ Python

[Anonymous]

Ich versuche, ein Skript zu schreiben, das die Codon-Verwendung in Sequenz analysiert, die das Codon-BIAS-Paket verwendet.

Code: Select all

    FOC = cb.scores.FrequencyOfOptimalCodons (ref_seq=sequence_list, genetic_code=11)

wobei Sequence_List eine Liste von STR -Objekten ist, die ORFs enthalten, und der Genetic_Code für bakterielle, archaeale und pflanzliche Plastid -Code,
Ich erhalte das Folgende aus meiner Shell:
Traceback (most recent call last):
File "I:\R&D\Product Research Group\Metabolic&Regulatory modeling\Codon usage\Python scripts\CodonBias CAI analyzer.py", line 117, in
codon_df, total_orfs, analyzed_orfs = analyze_codon_usage(orfs_file, trna_file)
File "I:\R&D\Product Research Group\Metabolic&Regulatory modeling\Codon usage\Python scripts\CodonBias CAI analyzer.py", line 71, in analyze_codon_usage
FOC = cb.scores.FrequencyOfOptimalCodons (ref_seq=sequence_list, genetic_code=11)
File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\codonbias\scores.py", line 199, in __init__
self.weights = self.weights.droplevel('aa')
File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\generic.py", line 943, in droplevel
new_labels = labels.droplevel(level)
File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\indexes\base.py", line 2155, in droplevel
levnums = sorted(self._get_level_number(lev) for lev in level)[::-1]
File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\indexes\base.py", line 2155, in
levnums = sorted(self._get_level_number(lev) for lev in level)[::-1]
File "C:\Users\shlomog\AppData\Local\Programs\Python\Python312\Lib\site-packages\pandas\core\indexes\multi.py", line 1660, in _get_level_number
raise ValueError(
ValueError: The name aa occurs multiple times, use a level number
< /code>
Irgendeine Idee, was ich falsch mache? Ich ließ das Programm vor dem Anruf die Sequenzen in Sequence_List ausdrucken, und sie erscheinen in der Reihenfolge.>